Butanol production from alginate using Saccharomyces cerevisiae

It involves the construction of a Saccharomyces cerevisiae strain capable of degrading alginate to use it as a carbon source for the synthesis of butanol from brown seaweed carbohydrates. This yeast shows a high tolerance to alcohol toxicity. The technology involves the design and construction of a recombinant S. cerevisiae strain that incorporates the alginate degradation pathway. In addition, it includes an alginate lyase secretion pathway (endolytic and Continue reading

Compressed Genomic File System

The Compressed Genomic File System consists of a file management system that keeps these documents in a compressed format using an optimized algorithm. The three fundamental characteristics that differentiate this system with other genome compressors are: (1) This tool is not only a compressor, but a file system, which allows a transparent use; (2) given its compression algorithm, its efficiency increases when data correspond to similar samples, such Continue reading

Atacama Cream: Active compound from the desert for skin protection

Different sources were explored in the Atacama desert to find microorganisms: soil, salt lakes, sediments and water. The bacteria found in the desert can resist high UV radiation and low water concentrations. This is possible by synthesizing compounds capable of binding to water molecules to prevent its loss, and a pigment that protects from UV radiation. Using these compounds, a line Continue reading

Antarctic Enzymes: Commercial cryophilic enzymes from Antarctic krill and bacteria

Set of polypeptides from genetically modified enzymes obtained from Antarctic microorganisms –bacteria and krill-, able to function at low temperatures. By using protein engineering, the activity and stability of the enzymes at low temperature was improved in comparison to commercial and native enzymes. Xylanase, subtilisin and trypsin have been sequenced and cloned into expression vectors to be synthesized from Escherichia coli. Market Continue reading

Seaweed antioxidants

Phlorotannins are polyphenolic compounds that are only found in brown algae and are known for their various bioactive properties, e.g. antioxidant, antimicrobial, anticancer, anti-diabetes, anti-HIV, etc. Thus they have a great potential to be applied as medicine or nutraceuticals. Unlike conventional methods of phlorotannin extraction, our solution is based on the use of active enzymes isolated from the same brown algae, which increases Continue reading

Nutraceutical Flour from Llaita as Substrate

The development of new natural food products with nutraceutical potential based on llaita flour. These products are highly nutritious, because the llaita flour is composed of essential amino acids, polyunsaturated fatty acids and does not produce or contain cyanotoxins. Llaita is a natural biomass from high Andean wetlands, formed by colonies of filamentous Nostoc commune cyanobacterium, which is suitable for human consumption and is a natural nutrient Continue reading

Tailings Wall Optimization for Mining

Technology to support the building of tailings walls using the same waste rocks from the mine to raise the dam. The wall design incorporated different parameters such as tailings fluid characteristics (viscosity, yield, stress), geometry (wall size, height, angle of the slope, width of the dam) and characteristics of the rock being used (shape and stone size), which are considered on a mathematical model to predict Continue reading

High performance recombinant peptide production system

The peptides (between 30 and 50 amino acids) cannot be produced by traditional recombinant methods, and their chemical synthesis is inefficient and costly. Our process presents a new platform for peptide synthesis using E. coli and the design of expression vectors including both a localization and purification signal. The recombinant expression system and high performance purification process were designed and successfully implemented. Continue reading

Bioadditive for Heavy Oils

Fuel additives are chemicals that are added to various fuels such as gasoline, diesel, jet fuel, kerosene and many others, in order to improve performance. Such additives are responsible for reducing the effects of corrosion caused by fuels and improve their combustion properties. The technology provided by this development is an additive for heavy oils, derived from biodiesel from the plant Continue reading

Hyaluronic Acid production from new raw materials

Construction of genetically modified strains of E. coli. The first strain, optimized by adaptive evolution in order to get higher specific growth rate with xylose as carbon source (commonly found in forest residues). The second strain was modified to degrade mannitol and alginate from brown algae, as a carbohydrate source. These bacteria were modified with the hasA gene from Streptococcus pyogenes to Continue reading